Screening of antibody producing cells Serial check on immune reactions towards chemicals, and newly developed pharmaceuticals Optimization of anti-tumour activities of dendritic cells and T-lymphocytes Control of guided vaccinations, e.g. HIV Diagnosis and prognostic analysis of autoimmune diseases, e.g. Diabetes, Multiple Sclerosis or Rheumatoid Arthritis Monitoring of immune therapy Control of de-sensibilization treatment of allergic diseases Prediction of rejection crisis in organ transplantation Analysis of stem cell function Determination of transfection efficacy in gene therapy Measuring secretory products of low frequency tissue, tumour and immune cells
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ELISpot (enzyme-linked immunosorbent spot) assays are highly sensitive immunoassays that measure the frequency of antigen-specific T cells by measuring the number of cytokine-secreting cells within the blood at a single cell level.